Open AccessImpact of cytogenetic and genomic aberrations of the kallikrein locus in ovarian cancer
Author(s) -
Bayani Jane,
Paliouras Miltiadis,
Planque Chris,
Shan Shan J.C.,
Graham Cassandra,
Squire Jeremy A.,
Diamandis Eleftherios P.
Publication year - 2008
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1016/j.molonc.2008.07.001
Subject(s) - biology , ovarian cancer , locus (genetics) , gene , comparative genomic hybridization , kallikrein , copy number variation , cancer research , gene dosage , chromosomal translocation , gene duplication , genetics , cancer , gene expression , genome , biochemistry , enzyme
The tissue kallikrein (KLK) genes are a new source for biomarkers in ovarian cancer. However, there has been no systematic analysis of copy number and structural rearrangements related to their protein expression. Chromosomal rearrangements and copy number changes of the KLK region were studied by FISH with protein levels measured by ELISA. Ovarian cancer and cell lines revealed the KLK region was subject to copy number imbalances or involved in unbalanced translocations and were associated with increased protein expression of KLKs 5, 6, 7, 8, 9, 10 and 11. In this initial study, we introduce the potential for long‐range chromosomal effects and copy number as a mechanism for the previously reported aberrant expression of many KLK genes in ovarian cancers.