Open AccessMolecular detection of CFFDNA for early laboratory diagnosis of X linked disorders carriers
Author(s) -
Mahmoud Alalfy,
Mohamed Amr Hussein Elnoury,
Osama Azmy,
M.A. Bebars,
G. Alsharnoubi,
Reham M. Dawood,
Omar Nagy,
A. Ibrahim
Publication year - 2017
Publication title -
middle east fertility society journal/middle east fertility society journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 18
eISSN - 2090-3251
pISSN - 1110-5690
DOI - 10.1016/j.mefs.2017.04.001
Subject(s) - cell free fetal dna , fetus , medicine , pregnancy , obstetrics , gestational age , prenatal diagnosis , gestation , gynecology , biology , genetics
Enhancement of DNA detection assays increase the use of non-invasive prenatal diagnosis that is consequently enhances the supervision plan of pregnant woman with genetic disorder expected babies.Aim of work: Current work was planed to examine plasma of pregnant women starting from 6th week pregnancy for Cell-free fetal DNA, to determine embryonic sex type with consequent prospect of detection of fetal anomalies such as aneuploidies.Method: Samples collected from 6th week till 11th week of pregnancy according to pregnancy age and tested using Real time PCR for determination of fetal sex.Results: Testing of samples resulted in detection of fetal sex starting from 6th week and the later the gestational age the better the result for detection of fetal sex, all results were confirmed by Ultrasound scan and neonatal outcome. Testing results revealed PCR detection for 58 males and 92 females with confusion in one fetus due to non identical twins