
Comparing the growth and the development of mouse pre-antral follicle in medium with PL (Platelet Layset) and with FBS
Author(s) -
Hassan Pazoki,
Hussein Eimani,
Farhad Farokhi,
Abdolhossein Shahverdi,
Reza Salman Yazdi,
Leila Sadat Tahaei
Publication year - 2015
Publication title -
middle east fertility society journal/middle east fertility society journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 18
eISSN - 2090-3251
pISSN - 1110-5690
DOI - 10.1016/j.mefs.2015.01.006
Subject(s) - folliculogenesis , andrology , antral follicle , oocyte , follicle , ovarian follicle , biology , chemistry , medicine , endocrinology , ovary , embryogenesis , microbiology and biotechnology , embryo
In recent years a great deal of effort has been made to improve the culture medium which can be used for different cell culturing and Folliculogenesis. PL (Platelet Layset) with a high percentage of growth factors and also microelements can be effective on the growth of follicle and oocyte. Material and method: Preantral follicles were collected from 12 to 14 days female NMRI mice and cultured in culture medium with a different supplement as the serum for 12 days. The different serums were, 5% and 10% FBS as the control groups and the experimental groups were enriched by 5% PL, 10% PL and a combination of 5% PL and 5% FBS. The growth and development of follicle and oocyte was monitored and also the amount of Estradiol (E2) and progesterone (P4) was detected in different mediums on days 9 and 12 of culturing. Result: Oocytes had a significant growth in all medium compared to day zero and reached to the size of a mature oocyte (p < 0.05). Although survival rate after one day had significant decrease in all medium compared to 5% FBS (p < 0.05), after 12 days of culturing, the best survival rate belonged to 5% PL. Progesterone secretion increased in experimental groups significantly (p < 0.05) while Estradiol secretion in 5% and 10% PL decreased in half of control groups. Discussion: This result demonstrates that PL can be a considerable supplement that can be added to the culture medium or a good replacement for FBS as serum used in Folliculogenesis