Open AccessIn vivo evaluation of ovine sperm/embryo ability in mediating transgenic lamb
Author(s) -
Shahin Eghbalsaied,
Heydar Amini,
Kamran Ghaedi,
Akbar Pirestani,
Pouya Nabizadeh
Publication year - 2015
Publication title -
middle east fertility society journal/middle east fertility society journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 18
eISSN - 2090-3251
pISSN - 1110-5690
DOI - 10.1016/j.mefs.2015.01.005
Subject(s) - transgenesis , sperm , embryo , transgene , andrology , biology , exogenous dna , fertilisation , embryo transfer , human fertilization , insemination , artificial insemination , reproductive technology , embryogenesis , microbiology and biotechnology , gene , genetics , pregnancy , medicine
Here, we evaluated the ovine embryo ability in DNA uptake as an alternate pathway for sperm-mediated transgenesis. EGFP carrying plasmid was intra-uterus injected through laparoscopy either five days following natural insemination or mixed with sperm cells at the time of fertilization. Excluding trivial false-positive PCR results, following stringent negative control confirmation, PCR analysis of various tissues from 7 generated lambs did not reveal the transgene transfer. Therefore, non-viral vector incubation with sperm or embryo was inefficient for ewe transgenesis