In vivo evaluation of ovine sperm/embryo ability in mediating transgenic lamb | Zendy

Shahin Eghbalsaied | Zendy; Hamid-Reza Amini | Zendy; Kamran Ghaedi | Zendy; Akbar Pirestani | Zendy; Pouya Nabizadeh | Zendy

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In vivo evaluation of ovine sperm/embryo ability in mediating transgenic lamb

Author(s) -

Shahin Eghbalsaied,

Hamid-Reza Amini,

Kamran Ghaedi,

Akbar Pirestani,

Pouya Nabizadeh

Publication year - 2015

Publication title -

middle east fertility society journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.322

H-Index - 18

eISSN - 2090-3251

pISSN - 1110-5690

DOI - 10.1016/j.mefs.2015.01.005

Subject(s) - transgenesis , sperm , embryo , transgene , andrology , biology , fertilisation , exogenous dna , human fertilization , embryo transfer , insemination , artificial insemination , reproductive technology , embryogenesis , microbiology and biotechnology , gene , genetics , pregnancy , medicine

Here, we evaluated the ovine embryo ability in DNA uptake as an alternate pathway for sperm-mediated transgenesis. EGFP carrying plasmid was intra-uterus injected through laparoscopy either five days following natural insemination or mixed with sperm cells at the time of fertilization. Excluding trivial false-positive PCR results, following stringent negative control confirmation, PCR analysis of various tissues from 7 generated lambs did not reveal the transgene transfer. Therefore, non-viral vector incubation with sperm or embryo was inefficient for ewe transgenesis

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