Open AccessEffects of sphingosine-1-phosphate on gene expression of two cell mouse embryos induced by C2-Ceramide
Author(s) -
Xujing Geng,
Lei Guo,
Weisen Zeng,
Li Ma,
XiangHong Ou,
Chen Luo,
Qisheng Song,
Hong Li
Publication year - 2014
Publication title -
middle east fertility society journal/middle east fertility society journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 18
eISSN - 2090-3251
pISSN - 1110-5690
DOI - 10.1016/j.mefs.2013.06.001
Subject(s) - ceramide , sphingosine 1 phosphate , sphingosine , embryo , gene , sphingolipid , chemistry , gene expression , microbiology and biotechnology , biology , biochemistry , apoptosis , receptor
Objective: As to understand the sphingosine-1-phosphate (S1P) on gene expression of the pre-implantation mouse embryo induced by C2-Ceramide (CED).Materials and methods: Global gene-expression profiling of two cell mouse embryo was performed using Affymetrix Gene Chip® Mouse Genome 430 2.0 Array.Results: Fifty-five genes were identified with significant expression changes that are functionally involved in the two cell embryo after treated by S1P and CED. Of these genes, 30 were up-regulated and 25 were down-regulated. Genes Atm, Cdkn1b and Fgrfr2 are identified to be involved in the protective role of S1P against the apoptotic signals induced by CED.Conclusions: This study provides a map of genes in the pre-implantation two cell mouse embryo. Further investigation based on these data will provide a better understanding of the effects of S1P on the pre-implantation embryos in other mammalian species, especially human