Open AccessMiR‐217 is involved in the carcinogenesis of gastric cancer by down‐regulating CDH1 expression
Author(s) -
Li Wei,
Gao YuQiang
Publication year - 2018
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/j.kjms.2018.02.003
Subject(s) - cdh1 , microrna , medicine , pathogenesis , cancer , carcinogenesis , microvesicles , cancer research , bioinformatics , gene , pathology , biology , genetics , cadherin , cell
GC is one of the most leading malignancies all over the world, and is also the leading cause of cancer‐related mortalities. At present, GC remains difficult to diagnose at an early stage. In this study, we first detected the expression of 9 selected miRNAs in the exosomes from 67 GC patients' circular exosomes and found 4 miRNAs level was significantly altered. Meanwhile, one out of 4 candidate miRNAs also had a higher expression in the GC tissue samples, and negative correlated with CDH1 expression. Predicted by bioinformatics tools, confirmed by dual luciferase assay and immunoblotting, we identified that CDH1 is a direct target of miR‐217. MiR‐217 overexpression enhanced gastric cancer cells proliferation, and reduced exosomal CDH1 level which can be delivered into microenvironment. In conclusion, we constructed the negative correlation between miR‐217 and CDH1 level in GC patients and cells; unveiled part of the miR‐217 function during the pathogenesis of GC. These findings may give insight into understanding the mechanism of GC pathogenesis and provide new biomarkers for clinical diagnosis.