Open AccessGinseng polysaccharide serves as a potential radiosensitizer through inducing apoptosis and autophagy in the treatment of osteosarcoma
Author(s) -
Zhang XiaoYu,
Sun Ke,
Zhu Qi,
Song Tao,
Liu Yang
Publication year - 2017
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/j.kjms.2017.07.001
Subject(s) - autophagy , radiosensitizer , medicine , apoptosis , ginseng , osteosarcoma , cancer research , radiosensitivity , programmed cell death , p38 mitogen activated protein kinases , western blot , ionizing radiation , pharmacology , radiation therapy , microbiology and biotechnology , phosphorylation , pathology , biology , biochemistry , mapk/erk pathway , irradiation , gene , physics , nuclear physics , alternative medicine
Recent studies have confirmed that the combined use of anti‐cancer drugs with ionizing radiation (IR) could improve the sensitivity of osteosarcoma (OS) cells. Therefore, it is necessary to identify potential effective drugs for the enhancement of IR‐radiosensitivity. In the current study, we found that 20, 10, 5, and 1 μM of ginseng polysaccharide (GPS) significantly suppressed MG‐63 cell viability with or without γ‐ray radiation in a dose‐ and time‐dependent manner. Strikingly, 20 μM of GPS combined with 5 Gy treatment suppressed colony formation capacity by nearly 13.75∼fold compared with IR treatment alone. Our results showed that GPS could markedly induce early apoptosis and autophagy in MG‐63 cells. A higher drug concentration and a greater exposure dose were directly associated with more apoptosis and autophagy in cells. Western blot analysis showed that GPS decreased the phosphorylation of p38 and AKT as well as the protein expression of Bax and cleaved‐caspase3. In summary, GPS inhibited proliferation and increased apoptosis and autophagic death in OS cells, indicating that GPS may be a potential effective auxiliary drug for improving the IR sensitivity of OS patients.