Open AccessGenetic diversity of multidrug‐resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU‐VNTR technique
Author(s) -
Khosravi Azar Dokht,
Shahraki Abdolrazagh Hashemi,
Dezfuli Soolmaz Khandan,
Hashemzadeh Mohammad,
Goodarzi Hamed,
Mohajeri Parviz
Publication year - 2017
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/j.kjms.2017.06.011
Subject(s) - typing , genotyping , tuberculosis , mycobacterium tuberculosis , genotype , medicine , isoniazid , multiple drug resistance , allele , drug resistance , biology , virology , microbiology and biotechnology , genetics , gene , pathology
Tuberculosis (TB) is considered as one of the most important infectious diseases in the world, and recent rise and spread of multidrug‐resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made the matter worsened. Due to the importance of TB prevalence in Iran, this study was designed to investigate the genetic diversity among MDR strains of MTB by MIRU‐VNTR typing scheme. A total of 88 drug resistant M. tuberculosis isolates belong to pulmonary TB cases were collected from several TB reference centers of Iran. Drug susceptibility testing for Isoniazid and Rifampin was performed using the agar proportion method and MDR isolates were underwent genotyping by using 12‐locus‐ based MIRU‐VNTR typing. On performing proportion method, 22 isolates were identified as MDR. By typing of MDR isolates using 12‐loci MIRU‐VNTR technique, high diversity were demonstrated in MDR strains and these were classified into 20 distinct MIRU‐VNTR genotypes. MIRU loci 10 and 26 were the most discriminatory loci with 8 and 7 alleles respectively; while MIRU loci 2, 20, 24 and 39 were found to be the least discriminatory with 1–2 alleles each. We noticed a mixed infection in isolate 53, as this isolate comprised simultaneous two alleles in MIRU loci 40, 10, 16 and 39. In conclusion, this result represents MIRU‐VNTR typing as a useful tool for studying genetic diversity of MDR‐MTB in regional settings, and will help the health sectors to construct a preventive program for MDR‐TB. Additionally, it can detect mixed infection which can facilitate management of treatment.