Open AccessBMP‐2 combined with salvianolic acid B promotes cardiomyocyte differentiation of rat bone marrow mesenchymal stem cells
Author(s) -
Lv Yang,
Gao ChenWei,
Liu Bo,
Wang HaoYu,
Wang HaiPing
Publication year - 2017
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/j.kjms.2017.06.006
Subject(s) - mesenchymal stem cell , medicine , bone marrow , in vitro , desmin , bone morphogenetic protein 2 , microbiology and biotechnology , andrology , immunology , immunohistochemistry , pathology , biochemistry , biology , vimentin
The present study tested the hypotheses that bone morphogenetic protein 2(BMP‐2) combined with salvianolic acid B(Sal‐B) enhance the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) towards cardiomyocytes in vitro . BMSCs were treated with BMP‐2 and Sal‐B, alone or in combination, for 72 h, then added new media (excluding inductive substance) and cultured for 4 weeks. The morphologic characteristics, surface antigens and mRNA expression of several transcription factors were also assessed. We found that they could all be identified by the positive staining for cardiomyocyte‐specific proteins, desmin and cardiac troponin T, in these cells. Furthermore, the mRNA expression of GATA‐4 and Nkx2.5 genes was slightly increased on day 7, enhanced on day 14 and decreased on day 28 while α‐MHC gene was not expressed on day 7, but expressed slightly on day 14 and enhanced on day 28. The expression of these cardiac‐specific markers in treatment groups were all significantly higher than those in the control group, respectively ( P < 0.05). Transmission electron microscopy showed that BMSCs in treatment groups all had myofilaments, z line‐like substances and mitochondria. Taken together, these results indicate that BMP‐2 combined with Sal‐B promotes myocardial differentiation of BMSCs, which may represent a potential therapeutic strategy for the treatment of ischemic heart disease.