Open AccessPostconditioning attenuates acute intestinal ischemia–reperfusion injury
Author(s) -
Sengul Ilker,
Sengul Demet,
Guler Osman,
Hasanoglu Adnan,
Urhan Mustafa Kemal,
Taner Ahmet Sukru,
VintenJohansen Jakob
Publication year - 2013
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/j.kjms.2012.08.021
Subject(s) - medicine , malondialdehyde , superior mesenteric artery , reperfusion injury , ischemia , intestinal mucosa , intestinal ischemia , anesthesia , lipid peroxidation , creatine kinase , oxidative stress
The aim of this study was to test the hypothesis that postconditioning (POC) would reduce the detrimental effects of the acute intestinal ischemia–reperfusion (I/R) compared to those of the abrupt onset of reperfusion. POC has a protective effect on intestinal I/R injury by inhibiting events in the early minutes of reperfusion in rats. Twenty‐four Wistar–Albino rats were subjected to the occlusion of superior mesenteric artery for 30 minutes, then reperfused for 120 minutes, and randomized to the four different modalities of POC: (1) control (no intervention); (2) POC‐3 (three cycles of 10 seconds of reperfusion–reocclusion, 1 minute total intervention); (3) POC‐6 (six cycles of 10 seconds of reperfusion–reocclusion, 2 minutes total intervention); and (4) sham operation (laparotomy only). The arterial blood samples [0.3 mL total creatine kinase (CK) and 0.6 mL malondialdehyde (MDA)] and the intestinal mucosal MDA were collected from each after reperfusion. POC, especially POC‐6, was effective in attenuating postischemic pathology by decreasing the intestinal tissue MDA levels, serum total CK activity, inflammation, and total histopathological injury scores. POC exerted a protective effect on the intestinal mucosa by reducing the mesenteric oxidant generation, lipid peroxidation, and neutrophil accumulation. The six‐cycle algorithm demonstrated the best protection.