Open AccessImpact of methomyl lannate on physiological parameters of the land snail Eobania vermiculata
Author(s) -
Abdelmonem M. Khalil
Publication year - 2016
Publication title -
journal of basic and applied zoology /journal of basic and applied zoology
Language(s) - English
Resource type - Journals
eISSN - 2090-9896
pISSN - 2090-990X
DOI - 10.1016/j.jobaz.2015.12.005
Subject(s) - methomyl , snail , land snail , biology , lactate dehydrogenase , alkaline phosphatase , hemolymph , toxicology , aspartate transaminase , microbiology and biotechnology , pesticide , veterinary medicine , biochemistry , enzyme , ecology , medicine
The methomyl lannate is a carbamate pesticide, which is widely used and has known toxic effects on non-target organisms including land snails. This research investigated the acute and sublethal effects of lannate on land snail Eobania vermiculata. LD50 value (102.32μgsnail−1) of lannate was determined 48h following topical application. Some biomarkers such as acetylcholinesterase (AChE), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) as well as micronuclei (MN) and bi-nucleated cells (BN) were detected following 14-d topical exposure to sublethal doses 5.53, 21.32 and 53.30μgsnail−1. The results showed that AChE inhibition in the cerebral ganglia was higher than that in the foot muscle. With the exception of ALP, a significant induction in hemolymph enzymes was detected in the snails exposed to 21.32 and 53.30μgsnail−1. Also, the results showed that (MN) frequencies of lannate treated snails were ∼3-, 4- and 6-fold that of the control in the snail exposed to 5.53, 21.32 and 53.30μgsnail−1, respectively. The observed changes in these biomarkers may provide useful information regarding environmental conditions and risk assessments of terrestrial organisms