Open AccessProtective effect of pumpkin seed oil against genotoxicity induced by azathioprine
Author(s) -
S. El-Fiky,
Ibrahim A. El-Elaimy,
Aziza M. Hassan,
Hany M. Ibrahim,
Reda Ismail Elsayad
Publication year - 2012
Publication title -
journal of basic and applied zoology /journal of basic and applied zoology
Language(s) - English
Resource type - Journals
eISSN - 2090-9896
pISSN - 2090-990X
DOI - 10.1016/j.jobaz.2012.10.010
Subject(s) - genotoxicity , azathioprine , rapd , dna fragmentation , chemistry , biology , botany , food science , toxicity , medicine , biochemistry , genetic diversity , disease , organic chemistry , apoptosis , population , environmental health , programmed cell death
Pumpkin is a leafy green vegetable; it belongs to the Cucurbitaceae family. Pumpkin seed oil supplementation can prevent changes in plasma lipids and blood pressure. The present study was conducted to evaluate the protective effect of pumpkin seed oil against cytotoxicity and genotoxicity of azathioprine. Oral administration of pumpkin seed oil either before or after treatment of azathioprine was effective in the reduction of the frequencies of Mn-PCEs, decreased the DNA fragmentation, total sperm abnormalities and significantly increased sperm count, percentage of PCEs, and enhanced the ratio of PCEs to NCEs. However, random amplified polymorphism of DNA (RAPD) showed distinct differences in animal groups intoxicated with azathioprine before and after pumpkin seed oil treatment, which reflected a DNA protective effect of pumpkin seed oil. Depletion of glutathione content in the testis was also observed in azathioprine treated mice, which was improved by an oral administration of pumpkin seed oil either before or after treatment with azathioprine