In-silico primer designing and PCR for detection of novel coronavirus-19 | Zendy

Shashi Kant Tiwari | Zendy; Ajay Kumar Singh | Zendy; Avinash Singh | Zendy

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In-silico primer designing and PCR for detection of novel coronavirus-19

Author(s) -

Shashi Kant Tiwari,

Ajay Kumar Singh,

Avinash Singh

Publication year - 2020

Publication title -

journal of infection and public health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.983

H-Index - 35

eISSN - 1876-035X

pISSN - 1876-0341

DOI - 10.1016/j.jiph.2020.10.010

Subject(s) - in silico , primer (cosmetics) , covid-19 , genome , virology , coronavirus , biology , computational biology , virus , pandemic , cross reactivity , gene , genetics , cross reactions , medicine , chemistry , antigen , infectious disease (medical specialty) , disease , organic chemistry , pathology , outbreak

Novel corona virus (2019-nCoV or COVID-19) pandemic has been considered as a major public health emergency in the world of this century. We synthesised noval 12 pairs of primers from the whole genome of COVID 19 virus by using In-silico approach. We observed primers have high binding capacity and no cross reactivity. These primers and probe may be useful for commercial development of one step RTPCR based kit for diagnosis of COVID 19.

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