Indirect organogenesis from various explants of Hildegardia populifolia (Roxb.) Schott & Endl. – A threatened tree species from Eastern Ghats of Tamil Nadu, India | Zendy

A. R. Lavanya | Zendy; S. Muthukrishnan | Zendy; M. Muthukumar | Zendy; J. H. Franklin Benjamin | Zendy; T. Senthil Kumar | Zendy; V. Kumaresan | Zendy; M. V. Rao | Zendy

Open Access

Indirect organogenesis from various explants of Hildegardia populifolia (Roxb.) Schott & Endl. – A threatened tree species from Eastern Ghats of Tamil Nadu, India

Author(s) -

A. R. Lavanya,

S. Muthukrishnan,

M. Muthukumar,

J. H. Franklin Benjamin,

T. Senthil Kumar,

V. Kumaresan,

M. V. Rao

Publication year - 2014

Publication title -

journal of genetic engineering and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.729

H-Index - 25

eISSN - 2090-5920

pISSN - 1687-157X

DOI - 10.1016/j.jgeb.2014.09.002

Subject(s) - explant culture , organogenesis , callus , murashige and skoog medium , biology , botany , petiole (insect anatomy) , horticulture , in vitro , genus , biochemistry , gene

Hildegardia species are an important resource for fiber industry. This investigation was conducted to develop a plant regeneration protocol for Hildegardia populifolia (Roxb.) Schott & Endl. via indirect organogenesis Callus was obtained from leaf, internode and petiole explants, among these explants internode explant gave best result on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). The highest percentage (100%) of regeneration was obtained with benzyladenine (BA) (2.0mg/l)+indole-3-acetic acid (IAA) (0.1mg/l)+glutamine (25mg/l)+thidiazuron (TDZ) (0.5mg/l) from internode explants. Shootlets were highly rooted on MS medium supplemented with 3.0mg/l indole-3-butyric acid (IBA). In vitro rooted seedlings were successfully acclimatized. This in vitro regeneration system will facilitate further development of reliable procedures for this genus

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