Facile method for determination of deoxycytidine kinase activity in biological milieus | Zendy

Wei-Hua Hao | Zendy; Li-Chieh Yang | Zendy; Jong-Jing Wang | Zendy; Chang-Shan Hsu | Zendy; L.-C. Chang | Zendy; Kuang-Yang Hsu | Zendy

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Facile method for determination of deoxycytidine kinase activity in biological milieus

Author(s) -

Wei-Hua Hao,

Li-Chieh Yang,

Jong-Jing Wang,

Chang-Shan Hsu,

L.-C. Chang,

Kuang-Yang Hsu

Publication year - 2013

Publication title -

journal of food and drug analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.277

H-Index - 54

eISSN - 2224-6614

pISSN - 1021-9498

DOI - 10.1016/j.jfda.2013.09.008

Subject(s) - deoxycytidine kinase , deoxycytidine , chemistry , chromatography , medicine , cancer , gemcitabine

A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism

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