Validation of Eupatorium triplinerve Vahl Leaves, a Skin Care Herb from East Kalimantan, Using a Melanin Biosynthesis Assay | Zendy

Enos Tangke Arung | Zendy; Harlinda Kuspradini | Zendy; Irawan Wijaya Kusuma | Zendy; Kuniyoshi Shimizu | Zendy; Ryuichiro Kondo | Zendy

Open Access

Validation of Eupatorium triplinerve Vahl Leaves, a Skin Care Herb from East Kalimantan, Using a Melanin Biosynthesis Assay

Author(s) -

Enos Tangke Arung,

Harlinda Kuspradini,

Irawan Wijaya Kusuma,

Kuniyoshi Shimizu,

Ryuichiro Kondo

Publication year - 2012

Publication title -

journal of acupuncture and meridian studies

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.374

H-Index - 32

eISSN - 2093-8152

pISSN - 2005-2901

DOI - 10.1016/j.jams.2012.01.003

Subject(s) - tyrosinase , eupatorium , melanin , traditional medicine , ic50 , skin whitening , botany , fractionation , herb , chemistry , biology , biochemistry , medicine , pharmacology , in vitro , chromatography , medicinal herbs , active ingredient , enzyme

In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360 μM (L-tyrosine was used as the substrate) and above 2840 μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780 μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.

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