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P4‐536: CEREBROSPINAL FLUID BIOMARKERS FOR AMYLOID AND TAU USING FULLY AUTOMATED ASSAYS: ASSOCIATIONS WITH NEUROPATHOLOGY
Author(s) -
Mattsson Niklas,
Grinberg Lea T.,
Jonsson Magnus,
Seeley William W.,
Spina Salvatore,
Janelidze Shorena,
Rosen Howard J.,
Boxer Adam L.,
La Joie Renaud,
Lesman-Segev Orit H.,
Iaccarino Leonardo,
Kollmorgen Gwendlyn,
Eichenlaub Udo,
Miller Bruce L.,
Hansson Oskar,
Rabinovici Gil D.
Publication year - 2019
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2019.08.083
Subject(s) - neuropathology , progressive supranuclear palsy , corticobasal degeneration , cerebrospinal fluid , dementia , medicine , pathology , frontotemporal dementia , alzheimer's disease , frontotemporal lobar degeneration , temporal lobe , disease , psychology , oncology , psychiatry , epilepsy
comparison of CSF biomarkers across studies and laboratories. After discarding the first 2 mL of CSF, collection of CSF directly into a LowBind FBT substantially reduces variation in measured Ab(1– 42) concentrations. Maximum durations for stable storage at room temperature and 4 C were 48 hours and 15 days, respectively. Transportation had a significant impact on Ab(1–42) concentrations. Particularly, measured Ab(1–42) concentrations were approximately 15% lower after horizontal transport compared with upright transport. This reduction was resolved by increasing tube filling volume from 1.5 to 3.0 mL, and alleviated using cooled transport (4 C). Results for Ab(1–40) were similar to Ab(1–42), while pTau and tTau were not significantly affected by pre-analytical factors. Conclusions: Following 2 years’ comprehensive analysis of pre-analytical factors, we have finalized a protocol that minimizes the need for post-collection sample processing (no mixing, inverting, centrifugation or tube transfer) while also minimizing systematic differences in measured CSF AD biomarker concentrations.