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P1‐263: IDENTIFYING FACTORS THAT PREDICT LUMBAR PUNCTURE COMPLICATIONS AND PROMOTE PARTICIPATION IN LONGITUDINAL STUDIES REQUIRING SERIAL CSF SAMPLING
Author(s) -
Day Gregory S.,
Sathyan Sushila,
Rappai Tracy
Publication year - 2019
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2019.06.818
Subject(s) - medicine , lumbar puncture , longitudinal study , body mass index , dementia , logistic regression , physical therapy , cerebrospinal fluid , gerontology , disease , pathology
screening. A number of experiments to assess the effectiveness of an automated system were conducted. Precision and accuracy of hand pipetting verses automated pipetting was tested using aliquots of control materials. Assays were conducted over a three-day period by separate individuals and by the automation. Plate washing and ECL addition was held constant and carried out by the automation for both groups. Covered verses uncovered incubation determined if evaporative loss caused differences on biomarker detection levels. Sample incubation times were tested by shortening the interval from the recommended maximum to determine if less time had effect on detection levels. Automation liquid classes were verified using a Mettler Toledo balance and Hamilton software. Gravimetric measurements were made under control conditions (room temperature, humidity and reagent temperature). Different sample volumes were tested determine the effect of freeze thaw on detection levels. Results: Although interestingly similar the automation pipetting profile was slightly different than the hand; automation was more precise. Covered verses uncovered incubation showed virtually no difference in detection levels. Shorting sample incubations times had little effect on detection levels. Gravimetric measurements of liquid classes showed excellent precision and accuracy. Sample volume size did influence detection levels, lower volumes showed a decrease in biomarker levels. Conclusions: Although some differences are seen testing automation verses hand processing, the significance is negligible. The automated diagnostic test for detection of AD would be a cost effect, easily implement solution and become the first in line test for AD.