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P2‐145: EFFECT OF APOE E4 AND BDNF VAL66MET POLYMORPHISM ON BDNF LEVELS AND COGNITIVE PERFORMANCE IN MILD COGNITIVE IMPAIRMENT PATIENTS
Author(s) -
Cechova Katerina,
Chmatalova Zuzana,
Markova Hana,
Vyhnalek Martin,
Nikolai Tomas,
Matuskova Veronika,
Laczó Jan,
Andel Ross,
Angelucci Francesco,
Matoska Vaclav,
Hort Jakub
Publication year - 2019
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2019.06.2552
Subject(s) - apolipoprotein e , brain derived neurotrophic factor , medicine , endocrinology , neurotrophic factors , psychology , disease , receptor
and protein expression of molecules involved in degenerative pathways related to AD such as autophagy, proteosomal pathway, oxidative stress, survival, apoptosis, AB levels and phosphorylation of tau protein among the different groups of fibroblasts. Results: We characterize the 12 fibroblasts lines, by karyotyping, DNA sequencing and perform proteomic analyses. We found activation in the GSK3B pathway, tau hyperphosphorylation, with an increase in total tau expression and CatD overexpression in fibroblasts from AD patients compared to healthy controls. Conclusions: The fibroblasts of individuals with Alzheimer’s disease represent a suitable in vitro model for further molecular studies on the pathogenic process of AD, that could be used for the search of biomarkers of the disease and to validate potential new therapies. Additionally, these cells could be reprogrammed into iPCS, that had a broad differentiation potential, from which is possible to generate neurons, that would recapitulate the pathogenesis of AD brain cells.