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P1‐284: BASELINE RECOVERY AND LONGITUDINAL STABILITY OF CEREBROSPINAL FLUID AMYLOID‐β PEPTIDES: AN EVALUATION OF COLLECTION METHODS, LONGITUDINAL ASSAY PERFORMANCE, AND BIOMARKER STABILITY OVER A 2‐YEAR PERIOD
Author(s) -
Schauer Stephen,
Wildsmith Kristin R.,
Rabe Christina,
Honigberg Lee
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.290
Subject(s) - biomarker , cerebrospinal fluid , peptide , chromatography , chemistry , guanidine , medicine , biochemistry
was shown to be < 0.5 pg/mL and the LoQ < 5 pg/mL. No high dose hook effect was observed for samples containing up to 30000 pg/mL of phospho-Tau181. Linearity was shown across the clinical relevant range. A method comparison study with the INNOTEST PHOSPHO-TAU-(181P) assay demonstrated a good correlation (r>0.90). Conclusions:Automation, the mono test cartridge principle, short throughput times, and instrument flexibility are key attributes of the LUMIPULSE G instrument series making it the ideal platform to fulfill today’s needs for rapid and accurate quantification of CSF biomarkers. The novel Lumipulse G pTau 181 assay (under development) shows good sensitivity and precision, correlates well with the INNOTEST assay and completes the routine CSF biomarker panel for AD on LUMIPULSE.