Premium
P4‐044: THE GCAD CLOUD‐BASED WORKFLOW FOR PROCESSING WHOLE EXOME AND WHOLE GENOME DATA FROM THE ALZHEIMER'S DISEASE SEQUENCING PROJECT
Author(s) -
Gangadharan Prabhakaran,
Leung Yuk Yee,
Valladares Otto,
Chou Yi-Fan,
Kuzma Amanda B.,
Cantwell Laura B.,
Qu Liming,
Lin Han-Jen,
Zhao Yi,
Malamon John Stephen,
Naj Adam C.,
Salerno William J.,
Schellenberg Gerard D.,
Wang Li-San
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.2446
Subject(s) - workflow , indel , computer science , cloud computing , pipeline (software) , reference genome , genome , exome sequencing , database , exome , computational biology , data mining , single nucleotide polymorphism , genetics , genotype , biology , operating system , gene , mutation
membership models to the transcriptome data for clustering genes and identified ones that were characterized in each cluster. We performed Gene Ontology analysis on the characteristic genes in each cluster to examine their biological functions. Results:We found 673 genes that were differentially expressed among the different Braak stages (10% false discovery rate).Among them, genes in clusters related to neurons were downregulated in Braak stage V–VI as expected. Their downregulation appears to be consistent with the timing of tau-related neuropathology spreading into frontal cortex. Interestingly, genes in a cluster related to myelination were greatly altered in the Braak stages III–IV or more. Conclusions: The transcriptome analysis of autopsy brains in this study revealed aberrant expression in genes associated with myelination, which may precede the appearance of neuropathological changes associatedwithAD.Our resultsmay imply thatmyelin alteration could be an early event linking with AD pathogenesis.