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F1‐03‐03: THE ROLE OF MENINGEAL LYMPHATICS IN ALZHEIMER‐RELATED AMYLOID PATHOLOGY
Author(s) -
Acosta Natalia,
Antila Salli,
Keuters Meike H.,
Scoyni Flavia,
Korhonen Antti-Ville,
Bister Nea,
Konttinen Henna,
Wojciechowski Sara,
Koistinaho Jari,
Alitalo Kari,
Malm Tarja,
Tanila Heikki
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.2313
Subject(s) - lymphatic system , pathology , genetically modified mouse , lymphatic vessel , hippocampus , transgene , central nervous system , medicine , immune system , cortex (anatomy) , immunohistochemistry , biology , neuroscience , immunology , endocrinology , cancer , gene , biochemistry , metastasis
veins. Methods: Fluorescently-tagged soluble fixable Ab40 was injected into the cisterna magna of adult male C57BL/6J mice (6–10 weeks or 24–30 month old) and mice were sacrificed at 5 or 30 minutes after injection (n1⁄43 /group). Coronal sections from all areas of the brain were immunostained for markers of basement membranes and smooth muscle actin, followed by confocal microscopy and analyses. Results: Fluorescently-tagged soluble fixable Ab40 was injected into the cisterna magna of adult male C57BL/6J mice (6–10 weeks or 24–30 month old) and mice were sacrificed at 5 or 30 minutes after injection (n1⁄43 /group). Coronal sections from all areas of the brain were immunostained for markers of basement membranes and smooth muscle actin, followed by confocal microscopy and analyses. Conclusions:These results indicate that the entry route of soluble Ab from the CSF is along the pial–glial basement membrane of arteries. By 30 minutes after intracisternal injection, Ab is found in the intramural periarterial drainage pathways, suggesting that soluble Ab reaches the parenchymal extracellular spaces and enters the normal IPAD pathways.