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P3‐260: ALTERATION OF PLASMA‐FREE AMINO ACIDS (PFAAS) AND AMINES COULD BE NOVEL BLOOD‐BASED BIOMARKERS FOR DETECTION OF ALZHEIMER'S DISEASE AND FUTURE PROGRESSION
Author(s) -
Yano Yuki,
Kawai Nobuhiro,
Muramatsu Takahiko,
Arashida Naoko,
Nishimoto Rumi,
Shimbo Kazutaka,
Kasuga Kensaku,
Miyashita Akinori,
Ikeuchi Takeshi
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.1620
Subject(s) - receiver operating characteristic , biomarker , medicine , apolipoprotein e , area under the curve , mini–mental state examination , cognitive impairment , disease , gastroenterology , chemistry , oncology , biochemistry
(i) P-value<0.05, (ii) fold change 1.5 and (iii) consistency variation tendency in three groups. After assessed by Ingenuity Pathway Analysis (IPA), we then performed parallel reaction monitoring (PRM) mass spectrometry to accurately quantify the remaining samples. Receiver Operating Characteristic was used to differentiate samples in the different groups. Results:A total of 35 candidate proteins could distinguish AD from MCI, and a total of 34 candidates could distinguish AD from NC, with an area under the ROC curve (AUC) above 0.7, but failed to distinguish MCI from NC according to the same criteria. Stratification by gender revealed improved differentiation between AD vs. NC, AD vs. MCI, and MCI vs. NC with satisfactory results of AUC 0.9, sensitivity 80% and specificity 80%. Conclusions: Gender difference may play an important role in the detection of plasma biomarkers for AD. For females, we recommend Complement C1r subcomponent, Inter-alpha-trypsin inhibitor heavy chain H4, Alpha-2-HS-glycoprotein, Insulin-like growth factor-binding protein complex acid labile subunit, Kininogen-1, Ficolin-2, Complement C3, Transthyretin, Syntaxin-binding protein 5 and Antithrombin-III to distinguish MCI from NC. For males, we recommend Kininogen-1, Glutathione peroxidase 3, Alpha-1-antitrypsin and Clusterin. Furthermore, Mesothelin, Phosphatidylcholine-sterol acyltransferase and Phosphatidylinositol-glycan-specific phospholipase D for females and Syntaxin-binding protein 5, Clusterin, and Alpha-1-antitrypsin et al. for males may predict the conversion from MCI to AD.