Premium
P3‐072: FEASIBILITY OF USING X‐RAY FLUORESCENCE IMAGING AND ABSORPTION SPECTROSCOPY TO EVALUATE IRON DISTRIBUTION AND OXIDATIVE STATE IN THE ALZHEIMER'S DISEASE HIPPOCAMPUS
Author(s) -
DiGiacomo Phillip,
Webb Samuel,
Goubran Maged,
Plowey Edward D.,
Rutt Brian S.,
Zeineh Michael
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.1428
Subject(s) - x ray absorption spectroscopy , chemistry , absorption (acoustics) , synchrotron , beamline , absorption spectroscopy , spectroscopy , analytical chemistry (journal) , synchrotron radiation , fluorescence , x ray fluorescence , materials science , optics , physics , beam (structure) , chromatography , quantum mechanics , composite material
(NanoSIMS) in a combined approach we term SILK-SIMS. Results: 1) In human AD brain, the incorporation of tracer into plaques in a participant with clinical stage AD dementia suggests that plaques can be dynamic with activity over days even in later stages of disease. 2) Tracer enrichment was highly variable and asymmetric with both quiescent and active nanometer sub-regions of tracer incorporation. 3) Neurons are hyper-metabolic cells compared to surrounding neuropil. We will demonstrate whether disturbed neuronal metabolism (a proxy for function) may be indicative of declining neuronal health and whether this is correlated with the presence of tau aggregates. Conclusions: SILK-SIMS studies will provide invaluable information on plaque dynamics and neuronal health, and tau aggregation in the normal and diseased brain. These studies will offer new avenues for investigation into pathological mechanisms of the disease, with implications for therapeutic development.