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The impact of preanalytical variables on measuring cerebrospinal fluid biomarkers for Alzheimer's disease diagnosis: A review
Author(s) -
Hansson Oskar,
Mikulskis Alvydas,
Fagan Anne M.,
Teunissen Charlotte,
Zetterberg Henrik,
Vanderstichele Hugo,
Molinuevo Jose Luis,
Shaw Leslie M.,
Vandijck Manu,
Verbeek Marcel M.,
Savage Mary,
Mattsson Niklas,
Lewczuk Piotr,
Batrla Richard,
Rutz Sandra,
Dean Robert A.,
Blennow Kaj
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.05.008
Subject(s) - cerebrospinal fluid , medicine , biomarker , protocol (science) , disease , intensive care medicine , amyloid β , pathology , chemistry , biochemistry , alternative medicine
Cerebrospinal fluid (CSF) biomarkers have the potential to improve the diagnostic accuracy of Alzheimer's disease, yet there is a lack of harmonized preanalytical CSF handling protocols. Methods This systematic review summarizes the current literature on the influence of preanalytical variables on CSF biomarker concentration. We evaluated the evidence for three core CSF biomarkers: β‐amyloid 42, total tau, and phosphorylated tau. Results The clinically important variables with the largest amount of conflicting data included the temperature at which samples are stored, the time nonfrozen samples can be stored, and possible effects of additives such as detergents, blood contamination, and centrifugation. Conversely, we discovered that there is consensus that tube material has a significant effect. Discussion A unified CSF handling protocol is recommended to reduce preanalytical variability and facilitate comparison of CSF biomarkers across studies and laboratories. In future, experiments should use a gold standard with fresh CSF collected in low binding tubes.