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[P1–173]: VDR SUPPRESSION AND CYP24 INDUCTION BY AMYLOID BETA MIGHT INVOLVE MIR‐27B‐3P, LET‐7A‐5P, MIR‐125B‐5P IN AMYLOID BETA‐TREATED CORTICAL NEURON CULTURES
Author(s) -
Dursun Erdinc,
Candas Esin,
Yilmazer Selma,
GezenAk Duygu
Publication year - 2017
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2017.06.240
Subject(s) - calcitriol receptor , vitamin d and neurology , medicine , endocrinology , amyloid (mycology) , beta (programming language) , vitamin , chemistry , neurodegeneration , amyloid precursor protein , biology , alzheimer's disease , inorganic chemistry , disease , computer science , programming language
period, 3-hour restraint stress, and 12-hour post stress period. To evaluate the effects of blocking the transactivation arm of GR signaling through GR dimerization, we crossed APP/PS1 mice with the glucocorticoid receptor dimerization deficient mouse model (GRdim), to create GRdim APP mice. We also studied the effects of pharmacologically blockingGR dimerization using Compound A, administered via reverse microdialysis before restraint stress. Results:We observed a significant difference between male and female mice in the percent increase in ISF Ab during acute stress, with female mice having a much greater and sustained increase in ISF Ab compared to males. ISF Ab levels in female GRdim APP mice did not rise dramatically during acute stress, and stayed near baseline during the post stress period. Compound A completely blocked the increase in ISF Ab induced by restraint stress in female mice. Conclusions: These results suggest that females are more sensitive to stress-induced increases in Ab, and that acute stress increases Ab in female mice through a GR dimerization dependent mechanism.