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[P3–241]: MRI FINDINGS ASSOCIATED WITH CIRCULATING VEGF AND STIE2 CONCENTRATIONS IN YOUNG AND MIDDLE‐AGED ADULTS IN THE FRAMINGHAM HEART STUDY
Author(s) -
Raman Mekala R.,
Himali Jayandra J.,
Conner Sarah C.,
DeCarli Charles S.,
Vasan Ramachandran S.,
Beiser Alexa S.,
Seshadri Sudha,
Satizabal Claudia L.
Publication year - 2017
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2017.06.1454
Subject(s) - medicine , framingham heart study , cardiology , vascular endothelial growth factor , hyperintensity , angiopoietin receptor , blood pressure , angiogenesis , white matter , framingham risk score , magnetic resonance imaging , disease , radiology , vegf receptors
patients with a Glasgow Coma Scale (GCS) of 9-15, 30 severe TBI patients with a GCS of 8 or less, and 30 normal serum as control. Results:The digital ELISAs measured GFAP and UCH-L1 with a limit of detection (LOD) of 0.11 pg/mL and 0.63 pg/mL and a lower limit of quantification (LLOQ) of 0.48 pg/mL and 0.98 pg/mL in neat serum, respectively. Dilutional linearity was evaluated for both calibrator spiked normal serum and pooled TBI positive samples using sample diluent, and ranged from 110% to 117% for GFAP and 114% to 125% for UCH-L1. The mean of spike recovery was 90% for both assays. Both intraand inter-assay coefficients of variation (CV) were demonstrated with less than 10%. These digital ELISAs were able to quantify all of the samples tested. Mean serum levels of both proteins were found to be significantly higher in mTBI patients than in control (p< 0.0001), and were further elevated in severe TBI patients versus controls andmTBI patients (p< 0.0001 for GFAP and p1⁄40.002 for UCH-L1). Analysis of receiver operating characteristic (ROC) curves showed both proteins to be promising biomarkers for diagnosis of mTBI with an area of 0.9733 (0.9401-1.006, 95% CI) for GFAP and 0.8456 (0.7216-0.9695, 95%CI) for UCH-L1. Conclusions: Digital ELISA assays with high sensitivity and accuracy in quantification of both serum GFAP and UCH-L1 provide potential utility in diagnosis of mTBI.