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[P3–134]: IMMUNOMODULATORY EFFECT OF CORD BLOOD‐DERIVED MULTIPOTENT STEM CELLS ON LYMPHOCYTES OF PATIENTS WITH ALZHEIMER's DISEASE
Author(s) -
Li Xiaohong,
He Yi,
Zhu Tianrui,
Li Heng,
Feng Zhang
Publication year - 2017
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2017.06.1345
Subject(s) - cord blood , immunology , phytohaemagglutinin , flow cytometry , cd8 , cytokine , stem cell , multipotent stem cell , inflammation , medicine , immune system , biology , microbiology and biotechnology , progenitor cell
Background: There is increasing evidence suggesting that Alzheimer’s disease (AD) pathogenesis is associated with immunological mechanisms, mostly due to an inflammatory response that exacerbates AD pathology. Our main objective was to verify the hypothesis that peripheral inflammation could be linked with main AD biomarkers (CSF Ab1-42 and Tau, hippocampal volume (HV) and Default Mode Network (DMN) functional connectivity (FC)). Methods: All images were acquired in a 3T scanner (Philips Achieva) from 25 healthy controls, 45 aMCI and 28 mild AD patients. DMN mask was used as a template to extract each patients FC value of the DMN sub regions. FreeSurfer software v.5.3. was used to calculate the HV. Blood samples were collected from all participants; CSF sample was collected from aMCI and AD patients. We used a BD Cytometric Bead Array (CBA) to quantify the following cytokines: IL-1b, IL-6, IL-10, IL-12 and TNF-a. Results: Considering all neuroimaging variables of the study (HV and subparts of DMN FC), we found significant differences between groups in HV (mild AD < aMCI and controls) and FC of the right temporal region (aMCI< controls). Regarding controls, we did not find differences between detected versus non-detected for any variable. The subgroup aMCI with detected IL-6, IL-12 and TNF-a has > FC in left parietal and left temporal region of DMN, in relation to non-detected. In mild AD patients, we found the opposite pattern: patients with detected IL-10, IL-12 and TNF-a has < FC in hippocampus and region temporal of DMN, in relation to non-detected. AD patients with detected IL-10 showed < CSF levels of Ab1-42 and we found that patients with detected IL-6, IL-10 and IL-12 showed presented larger HV. Conclusions: We found an association between systemic inflammation and AD neuroimaging biomarkers (HVendDMNFC) and Ab1-42. In addition, we found an opposite pattern in the FC measures between AD and aMCI concerning the detected cytokines: while aMCI patients had an increased FC in some subparts of DMN, mild AD patients presented a decreased FC in some subparts of DMN. Our findings may indicate a possible relationship between inflammation and principal biomarkers of AD.