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[P3–094]: RESOURCE OF MULTIPLEX AFRICAN AMERICAN FAMILIES FOR WHOLE‐GENOME SEQUENCING
Author(s) -
Cuccaro Michael L.,
Reitz Christiane,
Beecham Gary W.,
Cukier Holly N.,
Celis Katrina,
Deon Adams Larry,
Starks Takiyah,
Joseph Nancy,
Whitehead Patrice L.,
HamiltonNelson Kara L.,
ReyesDumeyer Dolly,
Byfield Grace,
Bennett David A.,
Rosenberg Roger N.,
Boeve Bradley F.,
Sweet Robert A.,
Cruchaga Carlos,
Haines Jonathan L.,
Vance Jeffery M.,
Byrd Goldie S.,
Mayeux Richard,
PericakVance Margaret A.
Publication year - 2017
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2017.06.1304
Subject(s) - african american , disease , socioeconomic status , gerontology , medicine , genetics , biology , population , environmental health , ethnology , history
identified X11L as an APP interacting protein (2). We have reported that X11L is expressed specifically in neurons (3), it regulates intracellular transport and metabolism of APP in vivo (4, 5), and the production and deposition of brain Ab in human type APP-Tg was enhanced in X11L-KO mouse (6). Taken together, X11L is involved in the cause of AD. Methods: To demonstrate whether X11L involves in the gene expressions related to AD onset, we tried to identify the ApoE4-X11L regulated genes. To identify them, comprehensive gene expression analyses by RNA-seq were performed using brains of WT, X11L-KO, human ApoE4 (hApoE4)KI, hApoE4-KI/X11L-KOmice. Results:We identified a group of genes whose expressions were regulated by ApoE4 and X11L. GO analysis revealed that these genes are involved in intracellular protein transport. We also analyzed the gene expressional differences between human subjects with ApoE3 and ApoE4 using publicly available data, and identified common genes observed in mouse brain analysis. Conclusions: These suggest that there is a set of genes regulated by ApoE4 and X11L, which are tightly correlated to the cause of AD.Wewill report further analyses of obtained data and will reveal how ApoE4 and X11L regulated genes are involved in the cause of AD. (1) Rhinn [2013] Nature 500, 4550); (2) Tomita [1999] J. Biol. Chem. 274, 2243-2254; (3)Motodate [2016] Brain Res. 1646, 227-234; (4) Sano [2006] J. Biol. Chem. 281, 37853-37860; (5) Saito [2008] J. Biol. Chem. 283, 3576335771; (6) Kondo [2010] Mol. Neurodegener. 5, 35