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P1‐163: Functional and Pathological Characterization of the Brain Microvasculature in a Rat Model of Alzheimer's Disease
Author(s) -
Joo Lewis I.,
Lai Aaron Y.,
Sled John G.,
McLaurin JoAnne,
Stefanovic Bojana
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.910
Subject(s) - pathology , genetically modified mouse , parenchyma , somatosensory system , perivascular space , biology , medicine , neuroscience , transgene , biochemistry , gene
emerged from groundbreaking discoveries in the past few years on content of extracellular vesicles(EVs). EVs have been demonstrated to facilitate horizontal transfer of miRNAs and proteins between cells without direct cell-to-cell contact. Based on this background, the objective of this study was to characterize EMVs from MSC and evaluate the neuroprotective actions of EVs against the deleterious effects caused by exposure of hippocampal neurons to ADDLs. Methods:EVs characterization was performed by Flow cytometry, ElectronMicroscopy and Nanoparticle Tracking Analysis.Neuroprotection against OS was verified by general oxidative stress indicator and EVs enzyme content by Oroboros oxygraph. Moreover, we evaluate MSC’s miRNA expression profile and released through EVs in Next Generation Sequencing and EV’s miRNA internalization in neurons. Results: Our results indicate that EVs have markers of MSC membrane, and its peak release between 3 and 5 hours after cell stress. The internalization rate of EVs in neurons is increased when the neurons are subjected to ADDLs and it is is followed by miRNAs transfer into the cell. The presence of EVs in culture protected neurons against OS generated by exposure to ADDLs to control levels. Our hypothesis that this protection is based on the presence of catalase in content of EVs, proven by Oroboros oxygraph. Conclusions:Obtained results show the potential of EVs in treatment against damage by OS in AD.