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P1‐110: Neuroprotective Role of 17B Estradiol Against Amyloid Beta Neurotoxicity in Synaptosomes of Aging Female Rats
Author(s) -
Kumar Pardeep,
Baquer Najma
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.858
Subject(s) - medicine , endocrinology , monoamine oxidase , lipid peroxidation , antioxidant , chemistry , melatonin , neurotoxicity , enzyme , biology , biochemistry , toxicity
roid receptor component and estrogen response of two rat serotonin cell lines in order to determine if they could be of future assistance in this matter. Methods:The cells were grown in a standard cell culture medium for 24 hrs with 80–90% confluency in T75 flasks. Cells were washed with cold PBS twice and harvested in Trizol reagent. Further RNA isolation was done and RNA further purified with Qiagen column. RNA was quantified with Agilent Bioanalyzer chip and Nano600. RNA was hybridized to U33A Affymetrix chip. Microarray was done at Gene microarray core shared resources facility of Oregon Health and Sciences University. Data was generated by exporting thru MAS5.0 suit. The data CEL files were imported to Genesifter software using GCRMA log platform. Data analysis was done comparing RN46A and B14 cell lines untreated and treated with 17-beta-estradiol as a group and pairwise. Further real time PCR validation of microarray data will be presented. Results:The microarray results will be presented to define molecular characteristics of both cell lines. Cluster analysis and gene Ontology will be presented in the poster. After hormonal treatment, the larger differences in gene expression were observed in the following clusters: 1) Metabolic Pathways (3.2); 2) Alzheimer’s disease (5.36); 3) Huntington disease (5.36); 4) Parkinson’s disease (6.12); 5) Oxidative phosphorylation (6.38); 6) Neuroactive ligand receptor binding (-12.03); 7) Cytokine receptor interactions (-6.03). Conclusions:We proved that RN46A and B14 cells as a unique cell model to use in mechanistic studies involving the serotoninergic system. The gene expression data generated will be useful to establish these cell models to understand mechanisms of drug and hormonal actions. RN46A and B14 can be used to evaluate drug effects on serotonin neurons. These cells potentiate effect of neuro-steroids on serotonin pathways and importance of serotonin neural system in neurodegenerative diseases.