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P1‐091: Protection from β‐Amyloid Peptide Toxicity in a Transgenic C Aenorhabditis Elegans Model by Phenserine, a Long‐Acting Acetylcholinesterase Inhibitor and Nigella Sativa Oil, an Alkaloid Antioxidant
Author(s) -
Arsalan Khadijah
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.839
Subject(s) - pharmacology , neuroprotection , acetylcholinesterase , transgene , antioxidant , caenorhabditis elegans , toxicity , acetylcholinesterase inhibitor , neurotoxicity , biology , chemistry , biochemistry , medicine , enzyme , gene
extract were evaluated for Acetylcholinesterase inhibition using in-silico and in-vitro methods. Methods: Insilico docking method is carried out using LeadIT and simulation for the were done by AMBER tool. The protein structure (amino acid sequence) of human AChE whose pdb id is (1B41) was used for the evaluation, whose active site residues are Ser,Asn, Glu and His. The crystal structure of AChE was downloaded from RCSB protein data bank. Herbal compounds used for docking study were Quercetin, Ellagic acid, Ascorbic acid, Cinnamic acid and Piperine. Results: Herbal compounds used for docking study were Quercetin, Ellagic acid, Ascorbic acid, Cinnamic acid and Piperine whose lowest binding energy (kcal/mol) was found to be -9.54 kcal/mol, 13.0 kcal/mol, -13.0 kcal/mol, -13.6 kcal/mol, -5.17 kcal/mol and their supporting IC50 values obtained through in-vitro enzyme inhibition studies were 91 mg/ml 6 0.12, 200 mg/ml 6 0.21, 195 mg/ml 60.43, 200 mg/ml 6 0.24, 185 mg/ml 6 0.56 respectively. Along with this RMSD and RMSF value are calculate through simulation at 20ns on NPT. Donepezil was used as a standard and its lowest binding energy was found to be -6.17 kcal/mol and its corresponding IC50 value was found to be 155 mg/ml6 0.11. Conclusions:This study revealed the acetylcholinesterase inhibition potential of different commercially available crude Plant extract.