Premium
P4‐057: Efficacy of Fluorescence‐Labelled Amyloid‐Beta1‐42 on Microglial Activation
Author(s) -
Guo Ling
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.2147
Subject(s) - microglia , trypan blue , glial fibrillary acidic protein , in vitro , reactive oxygen species , fluorescence , amyloid (mycology) , chemistry , microbiology and biotechnology , pathology , biology , biochemistry , immunohistochemistry , immunology , medicine , inflammation , quantum mechanics , physics
impairment (VCI). Several studies show that carriers of the Met allele have reduced cognition, memory, and smaller hippocampal volume compared to Val/Val carriers. However, other studies have failed to find this or have found the opposite. These equivocal findings may be related to the interaction between BDNF polymorphism and biological sex. For example, the Met allele confers increased risk for dementia in females, but not males. Thus, we examined whether biological sex interacts with BDNF polymorphism to influence brain health. Methods:We conducted a secondary analysis of cognitive and neuroimaging data from the control arm of a 6-month single-blind randomized controlled trial of aerobic exercise in older adults with subcortical VCI, a population at high risk of progression to dementia. Executive processes were measured at baseline and 6-months using: 1) Trail Making Test (TMT; set-shifting); 2) Digit Symbol Substitution Test (DSST; sustained-attention); 3) Stroop Test (selective-attention, conflict resolution). TotalWML (mm) were quantified at baseline using a semiautomated analysis pipeline. BDNF genotyping was performed with a TaqMan by-design assay. Results:Over the 6 month period, Met carriers performed better on the TMTand the DSST compared to Val/Val carriers in females only. Among Val/Val carriers, males outperformed females on the TMT. Among Met carriers, females outperformed males on the DSST. Performance on the Stroop was not significantly impacted by sex or genotype. In females, Met carriers tended to have greater WML load than Val/Val carriers, and the opposite was seen in males. Among Val/Val carriers, males had greater WML load at baseline than females. Conclusions: Our study provides new knowledge about how biological sex can interact with genotype to alter brain health in older adults at risk for dementia. Future studies will examine whether the BDNF polymorphism moderates exercise efficacy on cognitive health in a sexdependent manner.