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P3‐179: Development of an Indicator Cell Assay for Blood‐Based Diagnosis of Alzheimer’s Disease
Author(s) -
Smith Jennifer Joy,
Danziger Samuel A.,
Miller Leslie,
Singh Karanbir,
Peskind Elaine R.,
Li Gail,
Lipshutz Robert,
Aitchison John
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.1839
Subject(s) - disease , amyotrophic lateral sclerosis , induced pluripotent stem cell , medicine , biology , immunology , gene , genetics , embryonic stem cell
dead cell assay using mouse primary hippocampal culture. Ab40/42 solutions were treated with a series of phenol-triazoles in order to monitor aggregation changes by fluorescence using K114. Results: Ab40/42SEC isolates demonstrated unique spectral shifts when incubated with K114. Oligomeric Ab aggregates were more toxic in comparison to Ab fibrillar species. A series of phenol-triazole derivatives inhibit the formation of oligomers by promoting the formation of high molecular weight aggregates, suggesting they can regulate Ab-associated neurotoxicity, which can be monitored in real time by spectral fluorescence changes using functional probes. In a live-dead cell assay, several phenol-triazole derivatives demonstrated neuroprotective effects in mouse primary hippocampal culture. Conclusions:Our novel spectral analysis is capable of detecting differences in fluorescence intensity and spectral shifts in the presence of Ab40/42 oligomers or fibrils. A series of phenol-triazoles promotes less toxic high molecular weight aggregates, which can be monitored by K114 fluorescence.