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P3‐045: Novel Tricyclic Indole‐Based Compounds in the Prevention of Alzheimer’s Disease
Author(s) -
Gujral Sarbjeet Singh,
Nekkar Rao Praveen P.
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.1703
Subject(s) - butyrylcholinesterase , cholinesterase , chemistry , tricyclic , pharmacophore , pharmacology , indole test , acetylcholinesterase , donepezil , alzheimer's disease , galantamine , amyloid beta , rivastigmine , biochemistry , enzyme , stereochemistry , medicine , disease , aché , dementia , peptide
factor (BDNF) and neurodegenerative diseases such as Parkinson’s disease, Huntington’s disease, and Alzheimer’s disease (AD). Recombinant BDNF has protective functions on in vitro and in vivo AD models. Methods: We cultured primary rat neuron cell with 100nM Ab for preconditioning. MTT(2.5mg/ml) assay was performed to measure cell viability. Western blotting; We detected cell survivor pathway protein and measured mitochondria potential by using JC-1. Results: 100nM Ab42 Preconditioning enhanced cellular tolerance and increased cell viability than control group at 1mM Ab toxicity. Ab42 Preconditioning maintained mitochondria potential and prevented cell apoptosis. Conclusions:This study indicates that mild Ab Preconditioning activates cell survivor mechanism and prevents apoptosis result from Ab toxicity. This survivor maybe arise from BDNF pathway.