Premium
P2‐041: Potential Therapeutic System for Alzheimer’s Disease By Removal of Blood AB: Efficient Ab Removal System By Enhancing Adsorption on Hollow Fibers with Hemodiafiltration
Author(s) -
Morikawa Hiroshi,
Ohashi Norimi,
Kawaguchi Kazunori,
Sakai Kazuyoshi,
Nakai Shigeru,
Hiki Yoshiyuki,
Sobajima Hiroshi,
Ihira Masaru,
Kitaguchi Nobuya
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.1245
Subject(s) - adsorption , hemodialysis , chemistry , dialysis , filtration (mathematics) , chromatography , surgery , medicine , organic chemistry , statistics , mathematics
postmortem brains of 17 HD patients (mean age at death, 75. 7 6 10.4 years) and 16 age-matched non-HD subjects (79.0 6 12.6 years) were immunostained with the anti-Ab antibodies 4G8 (anti Ab17-24) and DE2 (anti Ab1-16); they were also subjected to silver staining by the periodic acid-methenamine-silver (PAM) and Gallyas-Braak (GB) methods, anti-phospho-PHF-Tau antibody (AT8), anti-glial-fibrillary-acidic-protein (GFAP) antibody for astrocytes, and anti-Iba1 antibody for microglial cells. Results: All cortices of the brains of HD patients exhibited significantly fewer senile plaques than those of non-HD subjects stained with antiAb antibodies. This significant difference was also observed by silver staining. The parietal lobes of HD patients contained significantly fewer plaques compared to those of non-HD subjects. The temporal, frontal, and occipital lobes of HD patients showed a similar tendency, although not significant. However, the hippocampus showed no clear tendency. The number of Iba1 positive cells in the brain significantly increased in HD patients. The results of AT8 immunostaining will be discussed. Conclusions: Ab deposition in the brain was significantly decreased in HD patients who underwent removal of blood Ab three times per week. Therefore, hemodialysis-induced Ab influx into the blood may at least partly be derived from the brain. It suggested that removal of blood Abmight reduce brain Ab burden. Acknowledgment: This work was partly supported by KAKENHI (20509008, 22500419 and 23500531) and Smoking Research Foundation.