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P1‐289: Near‐Infrared Imaging of β‐Amyloid Species/Plaques in Animal Model
Author(s) -
Wong Man Shing,
Li Hung-Wing,
Li Yinhui,
Ho See-Lok,
Xu Di
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2016.06.1039
Subject(s) - fluorescence , chemistry , in vivo , cationic polymerization , fluorescence lifetime imaging microscopy , biophysics , preclinical imaging , biology , organic chemistry , physics , microbiology and biotechnology , quantum mechanics
at age of 20-month-old were used. We simultaneously injected Shiga-Y5 and Shiga-X22 at a dose of 200 mg/kg into the tail vein of three mice. At 3-h after the injection, F chemical shift imaging was obtained using a 7-tesla MR scanner. Another three mice were killed by overdose of sodium pentobarbital and we removed the brain. Then, the concentrations of Ab oligomers were measured by enzyme-linked immunosorbent assay (ELISA). Results: The F MR signal of Shiga-Y5 appeared mainly in the olfactory bulb, the subcortical area, and the cerebellum. In contrast, the F MR signal of Shiga-X22 displayed predominantly in the olfactory bulb, the cortex, and the cerebellum. Then, we produced a subtracted image by subtracting F MR images of Shiga-X22 from that of Shiga-Y5. F MR signal in the subtracted image was located in the subcortical area. ELISA measurement showed that Ab oligomers uniformly distributed in the brain except for the brainstem, and there is no specific region showing the accumulation of Ab oligomers. In contrast, the level of insoluble Ab was significantly less in the subcortical area where the concentration of insoluble Ab was relatively low. The imbalance between relative high level of Ab oligomers and the less level of insoluble Ab in the subcortical area may contribute to intense F MR signal in the subtracted image. Conclusions:We simultaneously injected Shiga-Y5 and Shiga-X22 into the tail vein of APPswe/ PS1dE9 double transgenic mice, and then obtained an image in the brain by subtracting FMR images of Shiga-X22 from those of Shiga-Y5 to reflect the distribution of Ab oligomers.