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Mitochondrial DNA differentiates Alzheimer's disease from Creutzfeldt‐Jakob disease
Author(s) -
Podlesniy Petar,
Llorens Franc,
Golanska Ewa,
Sikorska Beata,
Liberski Pawel,
Zerr Inga,
Trullas Ramon
Publication year - 2016
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.12.011
Subject(s) - mitochondrial dna , cerebrospinal fluid , biomarker , disease , dementia , pathology , alzheimer's disease , digital polymerase chain reaction , pathophysiology , creutzfeldt jakob syndrome , medicine , polymerase chain reaction , biology , genetics , gene , prion protein
Low content of cell‐free mitochondrial DNA (mtDNA) in cerebrospinal fluid (CSF) is a biomarker of early stage Alzheimer's disease (AD), but whether mtDNA is altered in a rapid neurodegenerative dementia such as Creutzfeldt‐Jakob disease is unknown. Methods CSF mtDNA was measured using digital polymerase chain reaction (dPCR) in two independent cohorts comprising a total of 112 patients diagnosed with sporadic Creutzfeldt‐Jakob disease (sCJD), probable AD, or non‐Alzheimer's type dementia. Results Patients with AD exhibit low mtDNA content in CSF compared with patients diagnosed with sCJD or with non‐Alzheimer's type dementias. The CSF concentration of mtDNA does not correlate with Aβ, t‐tau, p‐tau, and 14‐3‐3 protein levels in CSF. Discussion Low‐CSF mtDNA is not a consequence of brain damage and allows the differential diagnosis of AD from sCJD and other dementias. These results support the hypothesis that mtDNA in CSF is a pathophysiological biomarker of AD.