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P3‐008: Alterations of the X chromosome in lymphocytes of Alzheimer disease patients
Author(s) -
Zivkovic Lada,
Bajic Vladan,
Milicevic Zorana,
Spremo-Potparevic Biljana
Publication year - 2015
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.06.874
Subject(s) - aneuploidy , chromosome instability , chromosome , biology , chromosome 21 , x chromosome , centromere , chromosome 9 , genetics , disease , pathology , medicine , gene
Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p<0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p<0.001). In addition, a strong (R2=0.97, n=20) and significant (p<0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.