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P2‐078: Neuroimaging and cerebrospinal fluid biomarkers in iraq and afghanistan veterans: Effects of deployment and blast concussion mild traumatic brain injury
Author(s) -
Peskind Elaine R.,
Petrie Eric C.,
Li Gail,
Cross Donna,
Yarnykh Vasily,
Richards Todd,
Minoshima Satoshi,
Mayer Cynthia,
Pagulayan Kathleen,
Huber Bertrand Russell,
Meabon James,
Raskind Murray A.,
Zhang Jing,
Cook David
Publication year - 2015
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.06.615
Subject(s) - concussion , traumatic brain injury , medicine , chronic traumatic encephalopathy , corpus callosum , white matter , fractional anisotropy , neuroimaging , psychiatry , poison control , magnetic resonance imaging , pathology , injury prevention , radiology , emergency medicine
in AD brains by iTRAQ-protein labeling and tandem mass spectrometry. Results: Compared to control samples, we identified more than 721 polypeptides, 61 of which were found over-expressed including Ferritin heavy chain, HSP70 and Phosphoglucomutase-1(PGM1). On the other hand, 71 proteins were found under expressed like RhoA, Sideroflexin-1 (SFXN1) and HSP60. To validate our results, we conducted semi-quantitative analyses with WB using specific antibodies against selected proteins. In addition, their homologue genes were manipulated in Drosophila models of Abetaand Tau-induced pathology to assess their specific involvement in the disease. After inspection of eye phenotypes in such fly models. Conclusions:We found that some proteins do not appear to modify Abeta or Tau insults. However, other proteins can either modify Abeta toxicity, Tau toxicity or both, suggesting specific interactions with different pathways. This approach illustrates the potential of Drosophila models to validate hits after mass spectrometry studies and suggest that model organisms must be included in the pipeline to identify relevant targets. We anticipate that the results of this work may have important therapeutic implications for AD and related disorders.