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P2‐019: Optimization of specific multiplex DNA primers to detect variable CLU genomic lesions in patients with Alzheimer's disease
Author(s) -
Giau Vo,
Bagyinszky Eva,
An Seong Soo,
Chan Kim Seung,
Kim SangYun
Publication year - 2015
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.06.555
Subject(s) - dbsnp , single nucleotide polymorphism , genetics , biology , clusterin , primer (cosmetics) , amplicon , gene , snp , snp genotyping , polymerase chain reaction , genotype , apoptosis , chemistry , organic chemistry
Background: Recently, polymorphisms in the clusterin (CLU) or Apolipoprotein J (Apo J) gene were reported to be involved in lipid metabolism, atherogenesis, and Alzheimer’s disease (AD). The influences from genetic variation had not been examined among Koreans. Methods: To have better understanding on a genome-wide analysis of fusion genes with efficient methods of correlation analyses, we developed PCR primer pairs for CLU gene. The new primer set can target specific regions of previously sequenced CLU gene. Primers were designed to target eight exon amplicons with flanking regions to optimize PCR amplification. One hundred samples from clinically diagnosed Korean dementia patients were selected for testing. Results:We identified four single nucleotide polymorphisms (SNPs) in CLU through direct sequencing of the PCR products. These included three SNPs (rs7982, rs2279590 and rs3216167) that were previously reported variants in the SNP database (dbSNP), which could be found in NCBI. Interestingly, one new (NEW1) or extremely low-frequency mutation was found in more than one individual among the late-onset AD subjects. Conclusions:Our study suggests that CLU variants may also be an AD susceptibility factor among Koreans.