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P1‐066: Evaluation of the effect of amyloid beta oligomers on neurotrophins and GSK‐3/creb signal transduction pathways
Author(s) -
Lopez-Toledo Gustavo,
Carmen Cardenas-Aguayo Maria,
Gomez-Virgilio Laura,
Luna-Muñoz José,
Meraz-Rios Marco Antonio
Publication year - 2015
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.06.263
Subject(s) - neurotrophin , signal transduction , chemistry , creb , microbiology and biotechnology , synaptogenesis , neurogenesis , amyloid beta , neurotrophic factors , neuroscience , receptor , peptide , biochemistry , biology , transcription factor , gene
rat brain by controlled pore glass chromatography. In addition, we performed proximity ligation assays (PLA) to determine presence of ADAM10 and BACE1 in synaptic vesicles in situ in murine primary hippocampal neurons. Results:Western blot showed a prominent enrichment of ADAM10 and BACE1 as well as APP-CTF protein levels in synaptic vesicles. PLA also showed close proximity of ADAM and BACE1 with the synaptic vesicle marker synaptophysin. ADAM10 activity was also enriched whereas BACE1 activity could not be detected in synaptic vesicles, possibly due to the presence of the a-secretase product sAPPa which has been shown to inhibit BACE1. Conclusions: We conclude that ADAM10 and BACE1 are present in synaptic vesicles but that it is possible that BACE1 is inactivated at this location.