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Relationship between hippocampal atrophy and neuropathology markers: A 7T MRI validation study of the EADC‐ADNI Harmonized Hippocampal Segmentation Protocol
Author(s) -
Apostolova Liana G.,
Zarow Chris,
Biado Kristina,
Hurtz Sona,
Boccardi Marina,
Somme Johanne,
Honarpisheh Hedieh,
Blanken Anna E.,
Brook Jenny,
Tung Spencer,
Kraft Emily,
Lo Darrick,
Ng Denise,
Alger Jeffry R.,
Vinters Harry V.,
Bocchetta Martina,
Duvernoy Henri,
Jack Clifford R.,
Frisoni Giovanni B.,
Bartzokis George,
Csernansky John G.,
Leon Mony J.,
deToledoMorrell Leyla,
Killiany Ronald J.,
Lehericy Stephane,
Malykhin Nikolai,
Pantel Johannes,
Pruessner Jens C.,
Soininen Hilkka,
Watson Craig
Publication year - 2015
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2015.01.001
Subject(s) - hippocampal formation , subiculum , neuropathology , cresyl violet , atrophy , pathology , hippocampus , alzheimer's disease , neuroscience , neuroimaging , biomarker , medicine , temporal lobe , psychology , disease , biology , dentate gyrus , staining , epilepsy , biochemistry
Objective The pathologic validation of European Alzheimer's Disease Consortium Alzheimer’s Disease Neuroimaging Initiative Center Harmonized Hippocampal Segmentation Protocol (HarP). Methods Temporal lobes of nine Alzheimer's disease (AD) and seven cognitively normal subjects were scanned post‐mortem at 7 Tesla. Hippocampal volumes were obtained with HarP. Six‐micrometer‐thick hippocampal slices were stained for amyloid beta (Aβ), tau, and cresyl violet. Hippocampal subfields were manually traced. Neuronal counts, Aβ, and tau burden for each hippocampal subfield were obtained. Results We found significant correlations between hippocampal volume and Braak and Braak staging (ρ = −0.75, P = .001), tau (ρ = −0.53, P = .034), Aβ burden (ρ = −0.61, P = .012), and neuronal count (ρ = 0.77, P < .001). Exploratory subfield‐wise significant associations were found for Aβ in Cornu Ammonis (CA)1 (ρ = −0.58, P = .019) and subiculum (ρ = −0.75, P = .001), tau in CA2 (ρ = −0.59, P = .016), and CA3 (ρ = −0.5, P = .047), and neuronal count in CA1 (ρ = 0.55, P = .028), CA3 (ρ = 0.65, P = .006), and CA4 (ρ = 0.76, P = .001). Conclusions The observed associations provide pathological confirmation of hippocampal morphometry as a valid biomarker for AD and pathologic validation of HarP.