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P1‐276: INVESTIGATION OF 99MTC‐LABELED CLIOQUINOL DERIVATIVE ON AMYLOID PLAQUE SPECIFICITY BY USING AN ANIMAL MODEL OF ALZHEIMER'S DISEASE
Author(s) -
Yurt KIlçar Ayfer,
Biber Müftüler Fazilet Zümrüt,
Evren Vedat,
Koylu Ersin Oguz,
Kanit Lutfiye,
Tekin Volkan,
Medine Emin Ilker,
Unak Perihan
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.05.516
Subject(s) - clioquinol , biodistribution , medicine , pathology , alzheimer's disease , β amyloid , hippocampus , senile plaques , disease , pharmacology , chemistry , biochemistry , in vitro
Objective: Alzheimer’s disease (AD), which comes up an important problem due to the aging population, has two hallmarks defined as amyloid plaques and neurofibrillary tangles. Despite the fact that AD affects a wide audience adversely, definitive diagnosis of AD still requires postmortem examination of the brain through histological staining of these hallmarks. Nowadays, these two hallmarks are playing crucial role in researches for diagnosis and treatment of AD. In addition, deficiency of AD diagnosis agents and limitation of assessing the blood-brain barrier (BBB) are main reasons of the current researches focusing on brain agents with plaque or tangle imaging potential. Metal-protein attenuating compounds (MPACs) such as clioquinol and cloxyquin have a great increasing promise on amyloid plaques. Yurt Kilcar et al. radiolabeled Bioquin-Hexa methyl propylene amine oxime (HMPAO) with 99mTc (99mTc-Bioquin-HMPAO) and evaluated the biodistribution on healthy male Balb/C mice (unpublished data). In current study, it is aimed to investigate amyloid plaque specifity of the 99mTc-BioquinHMPAO by using biodistribution studies on animal model of AD. Methods: Bioquin-HMPAO was labeled with 99mTc according to previous study. In accordance with the objectives of the study, animal model of AD created on healthy male Sprague Dawley rats with intrahippocampal stereotaxic injection of amyloid beta 1-42 (Aβ1-42). Intrahippocampal administration was applied to the CA1 area of hippocampus bilaterally (Aβ142 to the left side and PBS to the right side as control). Additionally, healthy male Sprague Dawley rats were utilized as naive group. Twenty days after surgery, biodistribution studies of 99mTc-Bioquin-HMPAO on animal model of AD and control group Susanne Granegger1, Lindsay Brammen2, Helmut Sinzinger1 1Institute ATHOS, Atherosclerosis Research, Vienna, Austria 2Vienna Medical University, Department of Nuclear Medicine, Vienna, Austria Elevated Lipoprotein(A) Impairs Platelet Radiolabeling Yield