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P1‐161: ROLE OF OXIDATIVE STRESS IN ALZHEIMER'S DISEASE AND ITS INFLUENCE ON P53 CONFORMATION
Author(s) -
Prandelli Chiara
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.05.399
Subject(s) - oxidative stress , disease , oxidative phosphorylation , neuroscience , oxidative damage , chemistry , biology , medicine , biochemistry
assay range were done using the same sample set. As the assay concept and procedure were unchanged, the other analytical performance parameters (e.g. Specificity, interfering substances, ...) were considered to be unaffected. Results: The Limit of Detection (LOD 1⁄4 mean blank + 2*Stdev) of the updated assay, based on 28 measurements of the blank (1⁄4 sample diluent) was determined to be 65.2 pg/ml. The intraand inter-assay variability were found to be 4.6% (range 0.8% 11.0%) and 7.8% (range 1.4% 18.0%) respectively based on the results of the CSF samples tested over four different runs. CSF sample concentrations determined with the updated product aligned well with those of the previous version. The assay range, defined by the lower limit of quantification and upper limit of quantification, ranged from 225 pg/ml to 1452 pg/ml, based on the results from the sample set described above. Conclusions: The updated INNOTEST b-Amyloid 1-42 performance data complied with the standardization requirements of the AD diagnostic community. The improvements to the product were assessed to preserve the clinical utility of the CSF Amyloid-b 1-42 results.