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P1‐154: QUANTITATIVE EEG (QEEG) AS A DIAGNOSTIC TOOL FOR ALZHEIMER'S DEMENTIA IN PATIENTS WITH DOWN SYNDROME
Author(s) -
Waldemar Gunhild,
Salem Lise Cronberg,
Kjær Troels Wesenberg,
Sabers Anne,
Musaeus Christian,
Nielsen Martin Nøhr,
Nielsen Anne Grete,
Waldemar Gunhild
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.05.392
Subject(s) - dementia , quantitative electroencephalography , electroencephalography , audiology , psychology , population , alzheimer's disease , psychiatry , medicine , disease , environmental health
coupled to a hybrid quadropole orbitrap mass analyzer. Functionality and specificity of the immunoprecipitation was validated using Western blotting. Using isotope labeled peptides a selected reaction monitoring (SRM) method was developed to monitor and quantitate their identified endogenous equivalents in CSF. Results: We have detected seven proteotypic LAMP2 peptides, however not isoform specific, in CSF using mass spectrometry. By the combination of mass spectrometry and immunoprecipitationwe have confirmed the presence of a LAMP2 fragment consisting of at least amino acid 46-351. Furthermore we have developed an SRM method with the ability to quantitate the level of LAMP2 in CSF. Conclusions: For the first time we have detected LAMP2 in CSF using mass spectrometry and further developed an SRM method to quantitate the level of LAMP2. The protein is presently being explored as a potential CSF biomarker for AD in a number of case-control cohorts.