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P1‐112: MONOAMINERGIC BRAIN TOPOCHEMISTRY IN ALZHEIMER'S DISEASE VERSUS HEALTHY ELDERLY
Author(s) -
Vermeiren Yannick,
Dam Debby,
Aerts Tony,
Engelborghs Sebastiaan,
De Deyn Peter Paul
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.05.349
Subject(s) - monoaminergic , homovanillic acid , neurochemical , dopaminergic , neuroscience , dopamine , monoamine neurotransmitter , serotonergic , psychology , medicine , hippocampus , population , neurodegeneration , alzheimer's disease , endocrinology , serotonin , disease , receptor , environmental health
particles in an in vitro model of the blood-brain barrier (BBB) consisting of porcine brain capillary endothelial cells. We here hypothesized that BCEC may express PLTP which may be involved in HDL metabolism in the brain. Methods: Immunohisto/cytochemical staining of porcine brain and cultured primary pBCEC.mRNA expression levels were determined by real-time PCR.PL transfer activity in pBCEC supernatants, cell lysates and in transwell supernatants was determined by radiometric assay.Results: PLTP is expressed in cerebrovascular endothelial cells PLTP expression and activity are up-regulated by LXR activation and in a polarized manner PLTP-mediated modification increases the cholesterol removal capacity of HDL 3 LXR activation up-regulates PLTP activity in vivo Endogenous PLTP remodels HDL and pre b-HDL formation is enhanced by LXR agonist PLTP silencing disrupts the biogenesis of HDL particles Conclusions: Phospholipid transfer protein is expressed in cerebrovascular endothelial cells and involved in HDL biogenesis and remodeling at the blood brain barrier.