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P4‐037: IMPROVEMENTS TO INNOTEST® PHOSPHO‐TAU 181P
Author(s) -
Darby Heather,
Leen Jef Vandijck Manu,
Decraemer Hilde,
De Smet Samantha,
Huyck Els,
Le Bastard Nathalie,
Kostanjevecki Vesna
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.05.1551
Subject(s) - replicate , diluent , chromatography , chemistry , multiplex , cerebrospinal fluid , analytical chemistry (journal) , mathematics , statistics , bioinformatics , medicine , biology , nuclear chemistry
replicates and technical replicates were analyzed for 3 AD and 3 non-AD over ten consecutive days. For the vast majority of the peptides CVs were <5%. The second, 6 AD and 6 non-AD, indicated slightly increased levels of cystatin C, beta-2-microglobulin, and APP, as well as clearly increased levels of chromogranin A, secretogranin-2, and neurosecretory protein VGF for AD. Moreover, for complex samples the advantage of high resolution instrumentation became evident because of the superior possiblity to avoid signal interferences. Conclusions: The assay is reproducible and consumes little amount per analysis. Preliminary results should be interpreted with caution due to the limited sample material. Data from a larger study, using clinically well characterized samples, is under way and will be presented.