O2‐06‐05: NEUTROPHILS INDUCE ALZHEIMER'S‐LIKE DISEASE VIA LFA‐1‐INTEGRIN AND NEUTROPHIL EXTRACELLULAR TRAPS

Methods: Confocal microscopy studies were performed to evaluate inflammation mechanisms. Intra-vital microscopy studies using two-photon microscopy were performed to visualize and analyze the movement of neutrophils inside brain vessels and in the parenchyma. In vitro rapid adhesion assays were performed on integrin ligands whereas and integrin affinity was measured using Image Stream technology. Neuropathological studies, fear conditioning and Y maze tests were performed to analyze the effect of inflammation mechanism inhibition on disease.