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O2‐06‐05: NEUTROPHILS INDUCE ALZHEIMER'S‐LIKE DISEASE VIA LFA‐1‐INTEGRIN AND NEUTROPHIL EXTRACELLULAR TRAPS
Author(s) -
Zenaro Elena,
Constantin Gabriela,
Pietronigro Enrica,
Della Bianca Vittorina,
Piacentino Gennj,
Turano Ermanna,
Montresor Alessio,
Laudanna Carlo,
Bonetti Bruno
Publication year - 2014
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2014.04.190
Subject(s) - neutrophil extracellular traps , intravital microscopy , extravasation , inflammation , integrin , microbiology and biotechnology , cell adhesion molecule , motility , serum amyloid p component , immunology , extracellular , chemistry , pathology , biology , medicine , microcirculation , receptor , biochemistry , c reactive protein
Methods: Confocal microscopy studies were performed to evaluate inflammation mechanisms. Intra-vital microscopy studies using two-photon microscopy were performed to visualize and analyze the movement of neutrophils inside brain vessels and in the parenchyma. In vitro rapid adhesion assays were performed on integrin ligands whereas and integrin affinity was measured using Image Stream technology. Neuropathological studies, fear conditioning and Y maze tests were performed to analyze the effect of inflammation mechanism inhibition on disease.