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P2–028: The novel calcium‐binding protein EFHD2 is linked to frontotemporal dementia
Author(s) -
Borger Eva,
GunnMoore Frank
Publication year - 2013
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2013.05.670
Subject(s) - frontotemporal dementia , synapse , neuroscience , immunocytochemistry , dementia , blot , immunostaining , biology , context (archaeology) , protein aggregation , microbiology and biotechnology , immunohistochemistry , pathology , medicine , disease , endocrinology , biochemistry , immunology , gene , paleontology
Notch-1 protein expression was decreased in the presence of HIF-1a siRNA, while DMOG, which is a specific revulsant of HIF-1a, increased the Notch1 protein expression further in both neurons and astrocytes. Interestingly, the expressions of HIF-1 a were lower in the presence of either g -secretase inhibitor DAPT or Notch-1 siRNA compared to that in the absence of the above two applications. The viability of the coculture cells was significantly lower in the ADDLs groups compared to the control groups, and the viability of the groups with the application of DMOG were worse further. While HIF-1a siRNA, DAPT or Notch-1 siRNA all improved the viability of coculture cells exposed to ADDLs respectively. Conclusions: Collectively, these results demonstrate that in the mixed coculture system of mouse hippocampal neurons and astrocytes, HIF-1a/Notch signaling pathway may mediate the apoptosis through an interactional way. M ore detailed studies are needed to investigate the molecular mechanism associated with the HIF1 a/Notch signaling pathway in AD